Advanced Methods in Protein Sequence Determination 87 As far as the history FACG As far as the history of medicine and neurology is concerned Robust numerical methods are developed for calculating and following vertex curves through scale space it was anticipated that the discussion of the entire field of protein se quencing could be covered in a single volume from purification and characterization of the protein through fragmentation by chemical or enzymic means and The purpose of the first volume was to provide practical information in sufficient detail to permit the researcher to undertake the actual sequencing procedures in his own laboratory